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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CFH
The concentration of Mouse TGFbI in the samples is then determined by comparing the OD of the samples to the standard curve
The concentration of Mouse C5a in the samples is then determined by comparing the OD of the samples to the standard curve
And then biotinylated Human HIF1AN Antibody is added and binds to HIF1AN in the sample
Human SYT13 (Synaptotagmin 13) ELISA Kit PC-III Standards or samples are addedThe Human (SYT13) Synaptotagmin 13 ELISA Kit measures Synaptotagmin 13 in samples. The plate has been pre coated with Human SYT13 antibody. SYT13 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human SYT13 Antibody is added and binds to SYT13 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated SYT13 antibody. After incubation unbound Streptavidin HRP is washed away during a
